| Title | CD26/dipeptidyl-peptidase IV in psoriatic skin: upregulation and topographical changes. |
|---|---|
| Published in | British Journal of Dermatology, Vol. vol. 158, p.p. 1264-p. 1272. |
| Author | Lingen, R.G. van; Kerkhof, P.C.M. van de; Seyger, M.M.B.; Jong, E.M.G.J. de; Rens, D.W.A. van; Poll, MK; Zeeuwen, P.L.J.M.; Erp, P.E.J. van |
| Date | 2008 |
| Type | article |
| Summary | BACKGROUND: Psoriasis is known to affect 2-3% of the population and can be considered an organ-specific autoimmune disease. CD26/dipeptidyl-peptidase IV (DPP-IV) is a membrane-bound protease with diverse properties. In theory, the expression of CD26/DPP-IV has common grounds with three principal key players of the psoriatic pathogenesis: keratinocytes, T cells and cytokines. OBJECTIVES: To assess CD26/DPP-IV expression in psoriasis in order to expand on the search for complementary biomarkers related to inflammation and proliferation in psoriasis. METHODS: The pattern of expression of CD26/DPP-IV was investigated on the mRNA-, protein- and enzyme-functionality level using immunohistochemical, immunofluorescent and enzyme activity labelling techniques. RESULTS: An 11-fold significant increase of CD26/DPP-IV on the mRNA level was demonstrated in psoriatic epidermal sheets compared with normal skin. Immunohistochemistry on psoriatic sections showed a distinct patchy honeycomb-like CD26/DPP-IV staining in the suprapapillary layers. Moreover, a clearly distinguishable column-like staining pattern throughout the suprabasal compartment along the rete ridges was seen, whereas in normal skin these patterns were absent. Strikingly, CD26/DPP-IV enzyme activity correlated with this immunohistochemical reactivity pattern for the CD26/DPP-IV protein. The T-cell bound expression of CD26/DPP-IV in psoriatic skin was explicitly present, albeit in small quantities. CONCLUSIONS: Our data provide clear evidence for a versatile upregulation of CD26/DPP-IV expression in psoriatic (epi)dermis. Although the exact functional contribution remains speculative, the topographical distribution of this complex multifunctional protein suggests a suitable role as a complementary biomarker in psoriasis. |
| Publication | http://repository.ubn.ru.nl/handle/2066/70195 |
| OpenURL | Search this publication in (your) library |
| Persistent Identifier | urn:nbn:nl:ui:22-2066/70195 |
| Metadata | XML |
| Repository | Radboud University Nijmegen |
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